Journal: Molecular Metabolism

Article Title: The mitochondrial pyruvate carrier regulates adipose glucose partitioning in female mice

doi: 10.1016/j.molmet.2024.102005

Figure Lengend Snippet: Adipose tissue MPC expression is altered in metabolically compromised mice and humans . ( A – B ) Normalized, quantified protein expression and representative western blot of mitochondrial pyruvate carrier proteins in subcutaneous adipose tissue from age-matched, chow fed wild type vs genetically obese (ob/ob) male mice ( A ) and from female and male human subjects with normal glucose tolerance vs impaired fasting glucose plus impaired glucose tolerance ( B ). ( C – H ) Pearson correlations between the protein expression of MPC1 ( A , D , E ) or MPC2 ( F , G , H ) in subcutaneous adipose tissue and indices of glucose tolerance ( C and F ), insulin sensitivity ( D and G ) and adipose insulin resistance ( E and H ) in human subjects separated by sex. Data are n = 5–8 in each group. ∗P < 0.05, ∗∗∗P < 0.001 vs control group ( A and B ).

Article Snippet: Immunoblot analysis was carried out on cell or adipose lysates using mouse or human primary antibodies against MPC1 (Sigma #HPA045119), MPC2 (Cell Signaling Technologies #46141), GAPDH (Sigma #G8795) and β-Tubulin (Abcam #179513) and developed by chemiluminescence (ECL).

Techniques: Expressing, Metabolic Labelling, Western Blot, Control